همنهشت نانوحامل‌های مزومتخلخل سیلیکا با زیست‌سازگاری بالا و توزیع منظم و یکنواخت حفره‌ها برای دارورسانی داروی دوکسوروبیسین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه صنعتی سهند- دانشکده مهندسی شیمی

2 دانشکده مهندسی شیمی، دانشگاه صنعتی سهند، تبریز، ایران

3 دانشگاه علوم پزشکی تبریز- دانشکده داروسازی و علوم نوین پزشکی

4 دانشگاه تبریز- دانشکده علوم طبیعی

5 دانشگاه تبریز- دانشکده دامپزشکی

چکیده

در این مطالعه، نانوحامل­های مزومتخلخل سیلیکای زیست‌سازگار همراه با توزیع منظم و یکنواخت حفره‌ها از منابع طبیعی هم‌چون سبوس برنج و گندم تحت فرایند سل- ژل برای دارورسانی پایدار به سلول­های سرطان سینه همنهشت شدند. خواص فیزیکی و شیمیایی نانوحامل­ها با تجزیه‌های  XRD،FT-IR، SEM و BET بررسی شد. نانوحامل‌های حاصل از سبوس برنج و گندم به‌ترتیب دارای مساحت سطح ویژۀ 741 و m2/g 630 همراه با توزیع منظم و یکنواخت حفره‌ها با اندازۀ 6/2 و nm 6/3 بودند. داروی دوکسوروبیسین به‌عنوان داروی مدل در نانوحامل‌ها بارگیری و رهش دارو در دو pH 4/7 و 4/5 بررسی شد. نتایج حاکی از دو برابر شدن نرخ رهش دارو در شرایط اسیدی شبیه­ساز محیط توموری بود.
با بررسی سمیت سلولی نانوحامل­ها روی رده­های سلولی HFF-2 و MCF-7، مشخص شد که نانوحامل­ها دارای زیست‌سازگاری بالا هستند و موجب جلوگیری از رشد و هم‌چنین مرگ سلول‌های سرطانی شده‌اند.

کلیدواژه‌ها


عنوان مقاله [English]

Synthesis of High Biocompatible Mesoporous Silica Nanocarriers with Regular and Uniform Pore Distribution for Doxorubicin Drug Delivery

نویسندگان [English]

  • S. Porrang 1
  • N. Rahemi 2
  • S. Davaran 3
  • M. Mahdavi 4
  • B. Hassanzadeh 5
1 Sahand University of Technology
2 Sahand University of Technology
3 Tabriz University of Medical Sciences
4 University of Tabriz
5 University of Tabriz
چکیده [English]

In this study, biocompatible mesoporous silica nanocarriers were synthesized with the regular and uniform pore distribution from natural sources of rice and wheat husk under sol-gel process for sustainable drug delivery to breast cancer cells. The physicochemical properties of nanocarriers were investigated by XRD, FT-IR, SEM and BET analyzes. Nanocarriers obtained from rice and wheat husk had a specific surface area of 741.44 and 630.52 m2/g, respectively, with regular and uniform pore distribution with a size of 2.58 and 3.63 nm. Doxorubicin was loaded as a model drug into the nanocarriers and drug release was evaluated at pH 7.4 and 5.4. The results showed that the drug release rate doubled under acidic conditions which simulating the tumor environment. By examining the cytotoxicity of nanocarriers on the HFF-2 and MCF-7 cell lines, it was found that the nanocarriers have high biocompatibility and prevent the growth and cause to cancerous cells death.

کلیدواژه‌ها [English]

  • Mesoporous Silica Nanoparticles
  • Regular Pore Distribution
  • Rice Husk
  • Wheat Husk
  • Drug Delivery
  • Breast Cancer
 
 
[1]        Ferrari, M., "Cancer nanotechnology: opportunities and challenges". Nature reviews cancer, 5,
pp. 161-171, (2005).
[2]        Liao, J., Jia, Y., Wu, Y., Shi, K., Yang, D., Li, P., Qian, Z., "Physical‐, chemical‐, and biological‐responsive nanomedicine for cancer therapy". Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 12, pp. e1581, (2020).
[3]        Narayan, R., Nayak, U., Raichur, A., Garg, S., "Mesoporous Silica Nanoparticles: A Comprehensive Review on Synthesis and Recent Advances". Pharmaceutics, 10, p. 118, (2018).
[4]        Slowing, I. I., Vivero-Escoto, J. L., Trewyn, B. G., Lin, V. S.-Y., "Mesoporous silica nanoparticles: structural design and applications". Journal of Materials Chemistry, 20, pp. 7924-7937, (2010).
[5]        Slowing, I. I., Vivero-Escoto, J. L., Wu, C. -W., Lin, V. S. -Y., "Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers". Advanced drug delivery reviews, 60,
pp. 1278-1288, (2008).
[6]        Souza, K., Ardisson, J., Sousa, E., "Study of mesoporous silica/magnetite systems in drug controlled release". Journal of Materials Science: Materials in Medicine, 20, p. 507, (2009).
[7]        Ke, J., Wang, Y., Wang, L., Yang, B., Gou, K., Qin, Y., Li, S., Li, H., "Synthesis and characterization of core-shell mesoporous silica nanoparticles with various shell thickness as indomethacin carriers: In vitro and in vivo evaluation". Microporous and Mesoporous Materials, 297, p. 110043, (2020).
[8]        Mishra, S., Rawal, A., Nebhani, L., "Imprinting the location of an in-built RAFT agent and selective grafting of polymer chains inside or outside the pores of mesoporous silica nanoparticles". Microporous and Mesoporous Materials, 294, p. 109898, (2020).
[9]        Slowing, I. I., Trewyn, B. G., Giri, S., Lin, V. Y. J. A. F. M., "Mesoporous silica nanoparticles for drug delivery and biosensing applications". 17,
pp. 1225-1236, (2007).
[10]      Gao, Y., Xiao, Y., Mao, K., Qin, X., Zhang, Y., Li, D., Zhang, Y., Li, J., Wan, H., He, S., "Thermoresponsive polymer-encapsulated hollow mesoporous silica nanoparticles and their application in insecticide delivery". Chemical Engineering Journal, 383, p. 123169, (2020).
[11]      Elgawady, Y., Ponnamma, D., Adham, S., Al-Maas, M., Ammar, A., Alamgir, K., Al-Maadeed, M. A. A., Hassan, M. K., "Mesoporous silica filled smart super oleophilic fibers of triblock copolymer nanocomposites for oil absorption applications". Emergent Materials, pp. 1-12, (2020).
[12]      Le, N. H., Hajjar-Garreau, S., Bonne, M.,
Megías-Sayago, C., Louis, B., Lebeau, B., Balan, L., "Photo-induced generation of size controlled Au nanoparticles on pure siliceous ordered mesoporous silica for catalytic applications". Microporous and Mesoporous Materials, 295, p. 109952, (2020).
[13]      Alam, Q., Hendrix, Y., Thijs, L., Lazaro, A., Schollbach, K., Brouwers, H., "Novel low temperature synthesis of sodium silicate and ordered mesoporous silica from incineration bottom ash". Journal of Cleaner Production, 211, pp. 874-883, (2019).
[14]      Lazaro, A., Quercia, G., Brouwers, H., Geus, J., "Synthesis of a green nano-silica material using beneficiated waste dunites and its application in concrete". World journal of nano science and engineering, pp. 41-51, (2013).
[15]      Tong, K. T., Vinai, R., Soutsos, M. N., "Use of Vietnamese rice husk ash for the production of sodium silicate as the activator for alkali-activated binders". Journal of cleaner production, 201,
pp. 272-286, (2018).
[16]      Chen, H., Wang, W., Martin, J. C., Oliphant, A. J., Doerr, P. A., Xu, J. F., DeBorn, K. M., Chen, C., Sun, L., "Extraction of lignocellulose and synthesis of porous silica nanoparticles from rice husks: a comprehensive utilization of rice husk biomass". ACS Sustainable Chemistry & Engineering, 1,
pp. 254-259, (2013).
[17]      Naqvi, J., Shah, F., Mansha, M., "Extraction of amorphous silica from wheat husk by using KMnO4". Journal of Faculty of Engineering & Technology, 18, pp. 39-46, (2011).
[18]      Renuka, N., Praveen, A., Anas, K., "Influence of CTAB molar ratio in tuning the texture of rice husk silica into MCM 41 and SBA-16". Materials Letters, 109, pp. 70-73, (2013).
[19]      Jang, H. T., Park, Y., Ko, Y. S., Lee, J. Y., Margandan, B., "Highly siliceous MCM-48 from rice husk ash for CO2 adsorption". International Journal of Greenhouse Gas Control, 3, pp. 545-549, (2009).
[20]      La-Salvia, N., Lovón-Quintana, J. J., Lovón, A. S. P., Valença, G. P., "Influence of aluminum addition in the framework of MCM-41 mesoporous molecular sieve synthesized by non-hydrothermal method
in an alkali-free system". Materials Research, 20,
pp. 1461-1469, (2017).
[21]      Wongsawang, C., Keawnuch, B., Busabok, C., "The Effect of Calcination Temperature on Crystal Structure of (Al, Cr) 2O3 Solid Solution". Science and Technology RMUTT Journal, 9, pp. 66-70, (2019).
[22]      Yan, F., Jiang, J., Chen, X., Tian, S., Li, K., "Synthesis and characterization of silica nanoparticles preparing by low-temperature vapor-phase hydrolysis of SiCl4". Industrial & Engineering Chemistry Research, 53, pp. 11884-11890, (2014).
[23]      Xu, H., Zhang, H., Wang, D., Wu, L., Liu, X., Jiao, Z., "A facile route for rapid synthesis of hollow mesoporous silica nanoparticles as pH-responsive delivery carrier". Journal of colloid and interface science, 451, pp. 101-107, (2015).
[24]      Yildirim, A., Bayindir, M., "A porosity difference based selective dissolution strategy to prepare shape-tailored hollow mesoporous silica nanoparticles". Journal of Materials Chemistry A., 3, pp. 3839-3846, (2015).
[25]      Zhao, W., Lang, M., Li, Y., Li, L., Shi, J., "Fabrication of uniform hollow mesoporous silica spheres and ellipsoids of tunable size through a facile hard-templating route". Journal of Materials Chemistry, 19, pp. 2778-2783, (2009).
 
[26]      Teng, Z., Wang, S., Su, X., Chen, G., Liu, Y., Luo, Z., Luo, W., Tang, Y., Ju, H., Zhao, D., "Facile synthesis of yolk–shell structured inorganic–organic hybrid spheres with ordered radial mesochannels". Advanced Materials, 26, pp. 3741-3747, (2014).
[27] Hu, X., Wei, W., Qi, X., Yu, H., Feng, L., Li, J., Wang, S., Zhang, J., Dong, W., "Preparation and characterization of a novel pH-sensitive Salecan-g-poly (acrylic acid) hydrogel for controlled release of doxorubicin". Journal of materials chemistry B., 3, pp. 2685-2697, (2015).