مهندسی شیمی ایران

مهندسی شیمی ایران

شبیه‌سازی، ارزیابی و بهبود تراشۀ ریزسیالی برای جداسازی اسپرم‌های متحرک

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشجوی کارشناسی مهندسی شیمی، دانشگاه صنعتی شریف
2 استادیار مهندسی شیمی، دانشگاه صنعتی شریف
3 دانشجوی کارشناسی ارشد مهندسی شیمی، دانشگاه صنعتی شریف
چکیده
سامانه­های ریزسیال، سیستم­هایی هستند که در آنها مقادیر بسیار کم سیال به‌وسیلۀ کانال­هایی در ابعاد میکرومتر کنترل می­شود. در این پژوهش، کارکرد یک سامانۀ ریزسیال برای جداسازی اسپرم‌های متحرک با شبیه­سازی به‌وسیلۀ روش دینامیک سیالات محاسباتی و استفادهاز ماژول جریان دوفازی، بررسی شده­است. این تراشه، دارای سه کانال ورودی و سه کانال خروجی است که در این هندسه، عرض کانال اصلی در انتهای کانال نسبتبه ابتدای آن افزایش یافتهاست. در ادامه، برای بهبود بازدهی تراشه، عرض کانال جمع­آوری بافر تغییر داده شدهاست تا از هدررفت بافر حاوی اسپرم­های متحرک جلوگیری شود و بازدهی جداسازی افزایش یابد. مشاهده­ شدهاست که با 4 برابرکردن عرض کانال خروجی بافر نسبت‌به عرض کانال­های ورودی، حجم بافر در کانال خروجی نمونه به 1 درصد کاهش پیدا می‌کند و این طرح بهعنوان طرح مناسب برای جداسازی اسپرم‌های متحرک انتخاب شده‌است.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Simulation, Assessment, and Improvement of a MicrofluidicChip Used for Motile-Sperms Separation

نویسندگان English

L. Dalir 1
S. Omidi 1
M. Saadatmand 2
F. Heidari 3
1 B. Sc. Student of Chemical Engineering, Sharif University of Technology
2 Assistant Professor of Chemical Engineering, Sharif University of Technology
3 M. Sc. Student of Chemical Engineering, Sharif University of Technology
چکیده English

Nowadays, infertility is one of the significant concerns of the World Health Organization (WHO), contributing to a large portion of its annual expenditure. Recent studies reveal that approximately 15% of couples worldwide (more than 50 million couples) encounter infertility problems that are caused by defects in the male or female fertility factors. Although medical advancements have successfully helped a lot of couples with their infertility by assisted reproductive technologies (ART), sperm selection, a crucial stage in ART, has remained challenging. Microfluidic devices are systems that control the movement of minute amounts of fluid using channels in micrometer dimensions, which have a wide range of applications, especially in medicine. This technology can help to enhancethe quality of sorted sperm and be applied to boost our knowledge of sperm behavior and effects of different sperm selection mechanisms within the ART. Here, the efficiency of a microfluidic device having an application in the separation of motile sperm was studied using computational fluid dynamics method and its two-phase-flow module. Laminar flow and Level-set modules were used in this simulation. The mentioned device has three input and three output channels, two channels for sample media and one for buffer. Furthermore, it has a main separation channel, which width becomes wider gradually from the beginning to the end. Thus, output channels have wider width. After simulation, the results were validated using the results of previous experimental and simulation works in this matter. Afterward, to increase the device’s efficiency, the width of the output channel used for
the buffer is being altered which prevents the wasting of the motile sperm in buffer. Simulation has shown that increasing the buffer output’s width by 4 times of input fluids channel’s width would cause decreasing the volume fraction of buffer in sample’s outlet to 1%. Therefore, this geometry is presented as the appropriate geometry for separation of motile sperm. In further studies, it is suggested that the last device be manufactured and studied to be validated its efficiency by experiment.

کلیدواژه‌ها English

Microfluidics
Infertility
Motile Sperm
Simulation
Two-Phase Flow
 
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