بررسی آزمایشگاهی تأثیر مایعات‌یونی بر میزان رشدمیکروارگانیسم های کاربردی در فرایندهای ازدیاد برداشت نفت و زیست‌پالایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مربی مهندسی شیمی، عضو هیئت علمی دانشگاه فنی و حرفه‌ای، گروه مهندسی شیمی، تهران، ایران

2 دانشجوی کارشناسی مهندسی تکنولوژی صنایع شیمیایی، دانشگاه فنی و حرفه‌ای، تهران، ایران

3 دانشجوی دکتری مهندسی شیمی، دانشگاه شیراز

4 کارشناس ارشد مهندسی شیمی، شرکت فناوری آتیه پویندگان اکسیر

چکیده

در این تحقیق برای نخستین بار، تأثیر مایعات یونی در غلظت‌ها و طول زنجیرۀ مختلف بر میزان رشد و عملکرد گونه‌های باکتریایی با قابلیت به‌کارگیری در فرایندهای ازدیاد برداشت ثالثیۀ میکروبی نفت، تجزیۀ زیستی و زیست‌پالایی بررسی شده است. در این راستا، دو مایع یونی با طول زنجیرۀ کربنی متفاوت با نام‌های 1- اوکتا‌دسیل-‌3‌- متیل ایمیدازولیوم‌کلرید و 1- دودسیل-3- متیل ایمیدازولیوم‌کلرید، سنتز شدند و پس از آن، تأثیر غلظت‌‌های متفاوت این مایعات یونی  ppm)0،10،25،50،100،200( بر 5 گونۀ (از خانوادۀ انتروباکتر و باسیلوس) جداشده از نفت و یا خاک‌ آلوده به نفت، بررسی شد. نتایج نشان داد که برای غلظت‌های بررسی‌شده، مقداری بهینه وجود دارد که در بیشتر مواردِ بررسی منجر به عملکرد و رشد بهتر میکروارگانیسم‌‌ها می‌شود. این غلظت، برای بیشتر گونه‌‌ها بین 10 الی   ppm25 و غلظت میکروب‌کش به‌دست‌آمده برابر با  ppm50 است؛ علاوه بر این، نتایج نشان می‌دهد که مایع یونی 1- اوکتا‌دسیل-‌3‌- متیل-‌ایمیدازولیوم کلرید در بیشتر موارد، عملکرد بهتری در افزایش رشد و فعالیت میکروارگانیسم‌‌‌ها، نسبت به 1- دودسیل-3- متیل ایمیدازولیوم‌کلرید، از خود نشان می‌دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Laboratory Study of the Effect of Ionic liquids on the Growth Rate of Microorganisms Applicable in the MEOR and Bioremediation Processes

نویسندگان [English]

  • P. Sarafzadeh 1
  • Z. Khosravi 2
  • F. Aram 3
  • Ali Zeinilabedini hezave 4
1 Instructor of Chemical Engineering, Faculty member of Technical and Vocational University (TVU), Tehran, Iran
2 B. Sc. Student of Industrial Chemical Engineering, Technical and Vocational University (TVU), Tehran, Iran
3 PhD Student of Chemical Engineering, Shiraz University
4 M. Sc. in Chemical Engineering, Fanavari Atiyeh Pouyandegan Exir Company
چکیده [English]

For the first time in this study, the effect of ionic liquids in different concentrations and properties on the growth and performance of microorganisms capable to be used for MEOR, biodegradation and bioremediation purposes has been investigated. In this regard, two ionic liquids with different characteristics and chain lengths, namely; 1-octadecyl-3-methylimidozalium chloride and 2-decyl-3-methylimidozalium chloride were synthesized in the laboratory and their effects were investigated in different concentrations (0, 10, 25, 50, 100, 200 ppm) on 5 different bacterial species (including Enterobacter and Bacillus families), isolated from oil or oil-contaminated soil. The results show an optimum amount of concentration on the top -down curve of the growth and performance of microorganisms in most cases. The maximum the growth rate was obtained for the most species, ranges between 10 and 25PPM and the antibacterial concentration of studied ionic liquids for all species was achieved in 50 ppm. Also, in most cases, 1-octadecyl-3-methylimidozalium chloride was shown to have a greater effect on the growth and yield of all microorganisms.

کلیدواژه‌ها [English]

  • Ionic Liquids
  • Microbial Enhanced Oil Recovery (MEOR)
  • Bioremediation
  • Biodegradation
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