Isolation of B cells using magnetic nanoparticles and investigating the effect of cell staining and the amount of nanoparticles on their isolation

Articles in Press

Document Type : Original Article

Author

Department of Polymer Engineering, Shiraz Branch, Islamic Azad University, Shiraz, Iran

10.22034/ijche.2024.423328.1364

Abstract

Today, magnetic nanoparticles and microparticles are used in various medical and therapeutic applications such as MRI, tissue repair, drug release, hyperthermia, and cell isolation. One of the most important cells to isolate are lymphoid cells, because they play a very important role in the treatment of lymphoma cancers and immunotherapy. Therefore, in this study, B cells were isolated from PBMCs using iron oxide magnetic nanoparticles coated with pectin. In order to isolate B cells, FITC anti-human CD20 antibody was attached to nanoparticles using EDC/NHS and analyzed by flow cytometry. In the next step, the target cells are separated from others cells with nanoparticles containing antibodies, and the rate of isolation is checked by flow cytometry. The results of flow cytometry show that the antibody is attached to the nanoparticles and its MFI is equal to 55. It was also found that by staining the cells after isolation, the reaction efficiency is somewhat reduced from 81% to 76% compared to the state without staining and direct analysis of the cells after washing by flow cytometry. In addition, the results showed that the appropriate amount of nanoparticles in the reaction with cells is 50 microliters and its reduction causes a 30% loss in the separation efficiency.

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Iranian Chemical Engineering Journal

Articles in Press, Accepted Manuscript
Available Online from 28 January 2024

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