Iranian Chemical Engineering Journal

Iranian Chemical Engineering Journal

Production of Yeast Extract Based on the Use of Beet Molasses in the Cultivation of Saccharomyces Cerevisiae

Document Type : Original Article

Authors
.S Kanjouri 1
Alireza Habibi 2
1 ,M. Sc. Student of Chemical Engineering, Razi University
2 Razi University, Faculty Of Engineering, Chemical Engineering Department
10.22034/ijche.2024.465405.1422
Abstract
This study aimed to reduce the production cost of yeast extract by the batch cultivation of Saccharomyces cerevisiae on beet molasses as the sole carbon source under the aerobic condition. The inhibitory effect of the substrate on yeast growth up to a concentration of 100 g L-1 was not observed and the Monod-kinetic model with constants μmax = 0.151 h-1 and KS = 10.30 g L-1 described perfectly the yeast growth. Also, in the cell maintenance energy modeling, the values of mS = 0.0518 g gcell-1 h-1 and YX/S,t = 1.739 g g-1 were determined which is in agreement with the high cell dense achievement in the fermentation stage. After fermentation, the processes of autolysis, plasmolysis, and ultrasound were studied for the disruption of cell-wall and the results showed that a combination of the plasmolysis process using 0.5 M NaCl and ultrasound at 20 kHz and 25 °C for 60 min was the best conditions to obtain the highest production efficiency of yeast extract from biomass (YP/X) of about 0.813 g g-1 with a protein purity percent (YP) of 72.8%.
Keywords
Industrial Microbiology
Microbial Growth Kinetics
Cell Wall Disruption
Autolysis
Plasmolysis
Ultrasound Waves
Subjects
 
 
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Iranian Chemical Engineering Journal
Volume 24, Issue 141
November and December 2025
Pages 19-33