Investigating the effect of solvent type on chlorophyll and carotenoid extraction from dry and wet microalgae

Articles in Press

Document Type : Original Article

Authors

Sahand University of Technology

10.22034/ijche.2023.369180.1251

Abstract

Chlorophyll and carotenoids can be extracted from microalgae using different solvents. In this study, for the first time, the extraction yield of pigments from mixed microalgae was compared in wet and dry conditions. The extraction yield of chlorophyll a, b and carotenoid using acetone solvent (without stirring) from wet algae equal 21.83%, 78.12%, and 66.01% respectively and from dry algae equals 61.34%,57.19%, and 53.27%, respectively. Then, to select the suitable solvent, acetone, ethanol, methanol, dimethyl ether, and tween (20% and 85% ) as non-ionic surfactants were used. Due to the difficult storage conditions of wet algae, dry algae was used in these experiments. The results show that the highest extraction yield of chlorophyll a, b, and carotenoid using tween (85%) without stirring on the tenth day is 91.70%, 87.12%, and 79.9% respectively. Also, the extraction yield with methanol solvent after 10 hours was 82.70%, 65.12%, and 62.90%. To reduce the extraction time using tween (85%), stirring was used. According to the results, the use of stirring increases the extraction rate of pigments, so that the extraction yield reached 98% within 50 min.

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Iranian Chemical Engineering Journal

Articles in Press, Accepted Manuscript
Available Online from 19 March 2023

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